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Projects

Bilayer lipid membranes on nanofabricated Si-chips

We use nano/micro-fabrication technology to exploit the molecular recognition and signal transduction properties of biomolecules and develop novel biomedical devices. Our main focus has been on the bilayer lipid membrane (BLM) which is the principal component of the cell membrane and is the platform for various cellular interactions. An ultra-stable, free-standing BLMs can be reconstructed using the nanofabricated Si-chip that we developed.

fig_blm

Ion channel sensors for high-throughput drug screening

Ion channels regulate the flow of ions across cell membranes and are thus the main target of drug discovery. We develop ion channel-based sensors for high-throughput drug screening by incorporating ion channel proteins to the ultra-stable BLM in the nanofabricated Si-chip. The system can also be used to study basic properties of ion channels in a defined condition.

fig1 fig2

In vitro reconstruction of neuronal networks

The aim of this project is to study the structure-function relationships of living neuronal networks in a well-defined system. We combine nanofabrication and surface engineering technologies to direct the growth of dissociated primary neurons and to design neuronal networks with a predetermined structure. Patch-clamp recordings and high-speed Ca imaging are the primary methods used to record neuronal activity. We also use computational modeling and graph theoretical analysis to theoretically verify the results and to make predictions for future experiments.

neuron